YACs Protocols

YACs related protocols

• Growth Of S. Cerevisiae And Preparation Of DNA (Subscription Required)

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot3298

• Large-Scale Preparation Of Agarose Plugs Of Yeast DNA (Subscription Required)

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4392

• Purification Of Yeast Artificial Chromosome (YAC) DNA With Filtration Units - Subscription Required

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot4393

• Restriction Digests Of High Molecular Weight Yeast DNA

  Source: Donis Keller lab manual - Washington University in St. Louis

• Restriction Digests Of YACs In Agarose Plugs

  Source: Donis Keller lab manual - Washington University in St. Louis

• Screening YAC Libraries

  Source: Donis Keller lab manual - Washington University in St. Louis

• Sizing Of YAC Clones

  Source: Donis Keller lab manual - Washington University in St. Louis

• Subcloning Of Yeast Artificial Chromosomes Into Phage Lambda

  Source: Donis Keller lab manual - Washington University in St. Louis

• Working With Yeast Artificial Chromosomes (Subscription Required)

  Source: CSH Protocols; 2006; doi:10.1101/pdb.prot3297

• YAC Transfer By KAR1

  Source: Koshland Lab, Carnegie Institution of Washington/Howard Hughes Medical Institute