Overview
There are two main types of detection methods for high-resolution FISH: conventional secondary antibody detection, and tyramide signal amplification (TSA). Conventional secondary antibodies for FISH are directly conjugated to fluorochromes, and recognize primary antibodies against probe labels. These fluorescently-conjugated secondary antibodies provide non-diffusible signals (4,5), but are usually much less sensitive than AP-based detection because they do not provide enzymatic signal amplification. The recently developed tyramide signal amplification (TSA) system removes this limitation by using horseradish-peroxidase (HRP)-conjugated antibodies to amplify the signals.
Details
Details of this protocol, FISH Protocols For Drosophila, are located on a web site other than Biocompare Protocols.