Overview
This protocol works for all stages except dauers (which won't open) and
hypoclorite-treated eggs (which disintegrate). Luckily, hypochlorite
treatment and fixation are by themselves sufficient to open eggs. This
technique should work for virtually all polyclonal sera most
monoclonals, as long as the major epitopes do not depend on disulfide
bonds. Animals fixed in this way can be stained with X-gal.
Mike Finney
Details
Details of this protocol, Fixation And Permeabilization Protocol For C. Elegans, are located on a web site other than Biocompare Protocols.