Overview
This assay is used when working with phage vectors carrying the beta-galactosidase gene (often used for immunological screening). If the cloning event disrupts a normally functional copy of the gene in the vector the resulting plaques would appear clear in the assay. If the phages contain a functional beta-galactosidase gene they will form blue rings around their plaques. Any strain which is not an overproducer of beta- galactosidase will work as indicator host bacteria; a single chromosomal copy of the gene is not a problem.
Details
Details of this protocol, Assay For Phage Containing The Beta-Galactosidase Gene, are located on a web site other than Biocompare Protocols.