Overview
To 2mls of mid-log culture or 1ml of freshly saturated culture add 1 ml(or an equal volume) of glycerol solution, mix gently, then freeze rapidly in liquid nitrogen or on dry ice. Store at -20°C and - 70°C.
Details
Details of this protocol, Bacterial Glycerol Stocks, are located on a web site other than Biocompare Protocols.