Overview
The gene knockout is one of the useful approaches to analyze the gene function. In C. elegans, the construction of deletion mutant library using chemical reagents and the subsequent PCR screening are generally used to isolate knockout mutants because the gene knockout using homologous recombination has not yet been developed.
Details
Details of this protocol, Gene Knockout Protocol By TMP/UV Method (Ver. 1.3), are located on a web site other than Biocompare Protocols.