Overview
A strain bearing an extrachromosomal array is irradiated with gamma rays or x-rays, several hundred F1 progeny carrying the array are picked to individual plates, and for each F1 several F2 carrying the array are picked to individual plates. These plates are screened for 100% transmission of the array to the F3; such a strain is homozygous for an integrated array.
Details
Details of this protocol, Integrating Extrachromasomal Arrays Into The C. Elegans Chromosomes, are located on a web site other than Biocompare Protocols.