Overview
Differential display of eukaryotic mRNAs has by now been employed as a research approach for almost three years in many laboratories5,7. It is a powerful technique which allows identification and cloning of those genes whose levels are subjected to changes upon specific circumstances. This technique enables us to detect genes whose RNA levels were changed. However, it is not possible to predict whether the levels of the corresponding proteins are actually modified. Since only a part of the transcript is amplified, the complete sequence data remains to be determined. Transcript amplification is not absolutely random, some RNA molecules will be preferably amplified over others according to their relative concentration and to whether they contain or not a sequence similar to the primer used for the DD.
Details
Details of this protocol, Optimized Welsh DDRT-PCR Protocol, are located on a web site other than Biocompare Protocols.