Overview
Here we report a rapid and convenient electroporation method for both gain- and loss-of function studies in vivo and in vitro in the rodent retina. Plasmid DNA directly injected into the subretinal space of neonatal rodent pups was taken up by a significant fraction of exposed cells following several pulses of high voltage. Using this technique, GFP expression vectors were efficiently transfected into retinal cells with little damage to the operated pups.
Details
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