Overview
Four techniques are described in this unit: scrape loading, scratch loading, bead loading, and syringe loading. Unlike microinjection, these techniques allow one to rapidly load (in a matter of minutes) thousands or even many millions of many types of mammalian cells with normally impermeant molecules, and so to facilitate quantitative analyses of the effect of loading.
Details
Details of this protocol, Direct Introduction Of Molecules Into Cells, are located on a web site other than Biocompare Protocols.