Overview
Genotyping Ts65Dn mice is based on doing simultaneous quantitative PCR amplification of a gene or genes in the Ts65Dn chromosome and a control gene on another chromosome (in this case Apob) and comparing the average change (delta) in threshold cycle (CT) between the Ts65Dn genes and the control gene. Doing a multiplexed reaction with an internal control avoids the need for determining DNA concentration and permits a relatively large range of variation in concentration of template DNA.
Details
Details of this protocol, Quantitative PCR Protocol, are located on a web site other than Biocompare Protocols.