Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization Protocol

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Protocol Information

Title Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
Source Martha F. Kramer and Donald M. Coen

Overview

This unit describes a method for amplifying DNA enzymatically by the polymerase chain reaction (PCR), including procedures to quickly determine conditions for successful amplification of the sequence and primer sets of interest, and to optimize for specificity, sensitivity, and yield.

Details

Details of this protocol, Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization, are located on a web site other than Biocompare Protocols.

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