Overview
This protocol describes nuclear and cytoplasmic fractionation of tissue culture cells and a method for Western blot detection of proteins using the Odyssey Infrared Imaging System. This protocol was used to detect expression of the "small" Tap protein in 293T, HeLa and COS cells. By Yukiko Misawa, Ying Li, David Rekosh and Marie-Louise Hammarskjd
Details
Details of this protocol, Western Blot Analysis Of Sub-Cellular Fractionated Samples Using The Odyssey Infrared Imaging System, are located on a web site other than Biocompare Protocols.