Overview
Reproducible detection of relevant autoantibodies in some human autoimmune diseases including multiple sclerosis (MS) has proven to be a difficult task, in part because commonly used techniques only permit detection of antibodies directed against linear epitopes on denatured proteins (Western blot) or do not adequately discriminate between denatured and folded proteins (ELISA).We have developed a method for the production of a series of multimeric antigens based on the ability of the streptavidin (SA) monomer to spontaneously assemble into a stable tetrameric structure. Multimerization of the antigen enables identification of antibodies that have a low binding affinity or are present at low concentrations. By Katherine McLaughlin, Kevin O'Connor, David Hafler, Kai Wucherpfennig and Please direct technical...
Details
Details of this protocol, Detection Of Autoantibodies With Self-Assembling Radiolabeled Antigen Tetramers, are located on a web site other than Biocompare Protocols.