Overview
Here we describe DNase-chip, an approach that can rapidly identify DNaseI HS sites for any region of interest, or potentially for the entire genome, by using tiled microarrays. We have used DNase-chip to identify DNaseI HS sites accurately from a representative 1% of the human genome in both primary and immortalized cell types. We find that although most HS sites are present in both cell types studied, some are cell type specific. This method can be applied globally or in a targeted fashion to any tissue from any species with a sequenced genome. By Crawford Gregory.
Details
Details of this protocol, DNase-Chip: A High Resolution Method To Identify DNaseI Hypersensitive Sites Using Tiled Microarrays, are located on a web site other than Biocompare Protocols.