Overview
Mast cells are now being understood as important intitiators and regulators in both innate and adaptive immunity in addition to their traditional roles in allergic and IgE-mediated immune responses.Transfection of primary leukocytes has traditionally been a challenging but much desired protocol. It allows not only the analysis of cells in a more natural state to a cell line system, it enables the direct comparison of, for example, transcriptional activity using luciferase reporters, in immune cells taken from genetically-altered mice. In addition, importantly it allows for "rescue experiments" in knockout cells as well as the ability to over-express or reconstitute wild-type and/or mutated constructs into wild-type or knockout cells for analysis of functional responses such as cytokine production, apoptosis, migration etc. By Kate Jeffrey Dr, Charles Mackay Prof and Tilman Brummer Dr
Details
Details of this protocol, Transfection Of Bone Marrow-Derived Mast Cells For Transcription Factor Luciferase Reporter Assays, are located on a web site other than Biocompare Protocols.