Overview
To test the effect of electric signals in vivo, we developed an alternative approach in addition to that achieved by using electrodes. Wounding the tissue instantly generates an endogenous electric current which could be measured directly(reference 4,6,7,22,47). Pharmacological or chemical agents which modulate ion transportation are applied to a wound to either enhance or decrease ion transportation, thus to enhance and decrease endogenous wound electric fields accordingly (reference 6,28,33,35). The effects of such modification on wound healing can be studied in vivo. Our protocols for applying drugs to modulate EFs are also provided. Cornea wound is used as a model system in this protocol. This protocol can be completed in 2 days. By Bing Song, Jin Pu, Brian Reid, Zhiqiang Zhao and Min Zhao
Details
Details of this protocol, Application Of Direct Current Electric Fields To Cells And Tissues In Vitro And Modulating Wound Electric Field In Vivo, are located on a web site other than Biocompare Protocols.