Overview
SecinH3 causes insulin resistance in mouse liver and in cell culture by inhibiting cytohesins that are effectors of the insulin signalling pathway. Stimulation with insulin leads to increased transcription of glycolytic genes, to suppressed transcription of gluconeogenetic genes and IGFBP1 in liver and in HepG2 cells. These insulin effects can be reverted by SecinH3. We quantified gene expression levels by real-time PCR. By Markus Hafner, Anton Schmitz and Michael Famulok
Details
Details of this protocol, Suppression Of Insulin Target Gene Expression By SecinH3 Quantified By Real-Time PCR, are located on a web site other than Biocompare Protocols.