Overview
Preparations of RNA containing an mRNA of interest are hybridized to a complementary single-stranded DNA probe. At the end of the reaction, nuclease S1 is used to degrade unhybridized regions of the probe, and the surviving DNA-RNA hybrids are then separated by gel electrophoresis and visualized by either autoradiography or Southern hybridization. The method can be used to quantitate RNAs, to map the positions of introns, and to identify the locations of 5' and 3' ends of mRNAs on cloned DNA templates. By Joseph Sambrook and David W. Russell.
Details
Details of this protocol, Mapping RNA With Nuclease S1 (Subscription Required), are located on a web site other than Biocompare Protocols.