Preparing Denatured Templates For Sequencing By Dideoxy-Mediated Chain Termination (Subscription Required) Protocol

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Protocol Information

Title Preparing Denatured Templates For Sequencing By Dideoxy-Mediated Chain Termination (Subscription Required)
Source CSH Protocols; 2006; doi:10.1101/pdb.prot3787

Overview

In this protocol, double-stranded plasmid DNA is denatured by alkali and annealed to an appropriate oligonucleotide primer, in preparation for dideoxy-sequencing reactions catalyzed by Sequenase.

Details

Details of this protocol, Preparing Denatured Templates For Sequencing By Dideoxy-Mediated Chain Termination (Subscription Required), are located on a web site other than Biocompare Protocols.

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