Overview
This protocol describes a procedure for uterine transfer, which is used for chimera production. The method is based on extensive work which resulted in the first successful development and birth of in-vitro-cultured mouse embryos. It is best to practice this procedure first on a cadaver and then on an anesthetized 2.5-days post coitum (dpc) pseudopregnant mouse using blue Affigel beads rather than embryos.
Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer
Details
Details of this protocol, Uterine Transfer (Subscription Required), are located on a web site other than Biocompare Protocols.