Overview
This protocol describes the standard method for nucleic acid purification by extraction first with phenol:chloroform (optionally containing hydroxyquiniline at 0.1%) and then with chloroform to remove any remaining phenol. The procedure takes advantage of the fact that deproteinization is more efficient when two different organic solvents are used instead of one. By Joseph Sambrook and David W. Russell.
Details
Details of this protocol, Purification Of Nucleic Acids By Extraction With Phenol:Chloroform (Subscription Required), are located on a web site other than Biocompare Protocols.