Overview
This protocol, involves lysis of cells in a monophasic solution of guanidine isothiocyanate and phenol. Addition of chloroform generates a second (organic) phase into which DNA and proteins are extracted, leaving RNA in the aqueous supernatant. The yield of total RNA depends on the tissue or cell source, but it is generally in the range of 4-7 µg/mg starting tissue or 5-10 µg/106 cells. By Joseph Sambrook and David W. Russell.
Details
Details of this protocol, A Single-Step Method For The Simultaneous Preparation Of DNA, RNA, And Protein From Cells And Tissues (Subscription Required), are located on a web site other than Biocompare Protocols.