Overview
The goal of this method is to identify transcriptionally active genes in cloned segments of genomic DNA. The protocol uses hybridization and affinity purification to recover biotin-labeled cDNAs that bind to a 500-kb segment of human DNA cloned in a BAC vector. However, the method can be easily adapted to other clones of genomic DNAs cloned in high-capacity vectors. This protocol was provided by Michael Lovett (Morgan et al. 1992; Simmons and Lovett 1999).
Details
Details of this protocol, Direct Selection Of CDNAs With Large Genomic DNA Clones (Subscription Required), are located on a web site other than Biocompare Protocols.