Overview
Uracil-containing bacteriophage M13 DNA, generated in Preparation of Uracil-containing Single-stranded Bacteriophage M13 DNA, is used in the classic double-primer method of oligonucleotide-mediated mutagenesis of Zoller and Smith (1984, 1987) to generate a heteroduplex molecule with uracil in the template strand and thymine in the strand synthesized in the in vitro reaction. Transformation of this DNA into an ung+ strain results in destruction of the template strand, with consequent suppression of production of wild-type bacteriophages. By Joseph Sambrook and David W. Russell
Details
Details of this protocol, Oligonucleotide-Directed Mutagenesis Of Single-Stranded DNA (Subscription Required), are located on a web site other than Biocompare Protocols.