Overview
Removal of the 5'-phosphate groups from the internal termini of bacteriophage {lambda} arms is used to prevent self-ligation and suppress the background of nonrecombinant bacteriophages during cloning. By Joseph Sambrook and David W. Russell.
Details
Details of this protocol, Alkaline Phosphatase Treatment Of Bacteriophage {lambda} Vector DNA (Subscription Required), are located on a web site other than Biocompare Protocols.