Overview
This protocol describes methods for isolation of DNA from a strain of S. cerevisiae carrying a recombinant YAC. Because the linear YAC DNAs are sensitive to shearing forces, pipettes with wide-bore tips should be used to transfer DNAs. The method is suitable for preparing DNA that will be used for agarose gel electrophoresis, Southern blotting, subcloning, genomic library construction, PCR, or other methods that do not require intact high-molecular-weight DNA. The expected yield from a 10-ml culture is 2-4 µg of yeast DNA.
Details
Details of this protocol, Growth Of S. Cerevisiae And Preparation Of DNA (Subscription Required), are located on a web site other than Biocompare Protocols.