Construction Of CDNA Libraries - Stage 4: Attachment Of Linkers Or Adaptors (Subscription Required) Protocol

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Title	Construction Of CDNA Libraries - Stage 4: Attachment Of Linkers Or Adaptors (Subscription Required)
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot3310

Overview

This stage achieves four goals: polishing the ends of double-stranded DNA, ligation of synthetic linkers or adaptors, digestion of the attached linkers to create cohesive termini, and preparing the cDNA for cloning. By Joseph Sambrook and David W. Russell.

Details

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