Overview
A detailed Protocol/Guide for the generation of Recombinant Adenoviruses. They report a strategy which simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.
Details
Details of this protocol, A Simplified System For Rapid Generation Of Recombinant Adenoviruses, are located on a web site other than Biocompare Protocols.