Overview
The inclusion of formamide in sequencing gels eliminates secondary structure in the DNA during electrophoresis. Formamide gels are particularly useful and almost a necessity when sequencing DNA templates with a G/C content >55%. This protocol requires all of the reagents listed in Preparation of Denaturing Polyacrylamide Gels.
Joseph Sambrook and David W. Russell
Details
Details of this protocol, Preparation Of Denaturing Polyacrylamide Gels Containing Formamide (Subscription Required), are located on a web site other than Biocompare Protocols.