Overview
This protocol is a modification of that outlined by Chirgwin et al. (1979). Tissues are homogenized in guanidinium isothiocyanate lysis buffer and then subjected to cesium chloride ultracentrifugation. The resulting RNA is then extracted with phenol and chloroform to remove protein and lipid contaminants. By Michael A. Connolly, Peter A. Clausen and James G. Lazar.
Details
Details of this protocol, Preparation Of RNA From Plant Tissue Using Guanidinium Isothiocyanate/Cesium Chloride Ultracentrifugation (Subscription Required), are located on a web site other than Biocompare Protocols.