Overview
The activity of ß-glucuronidase (GUS) can be accurately determined in intact plant tissue using 4-methylumbelliferyl ß-D-glucuronide (4-MUG) as a substrate. Upon hydrolysis by GUS, the fluorochrome 4-methyl umbelliferone (4-MU) is produced. This method is based on the permeability of both 4-MUG and 4-MU through plant tissue. By Miguel Blázquez.
Details
Details of this protocol, Quantitative GUS Activity Assay In Intact Plant Tissue (Subscription Required), are located on a web site other than Biocompare Protocols.