Overview
The first step in competitive RT-PCR is the synthesis and purification of the synthetic competitor. This is an RNA molecule designed to be reverse-transcribed and PCR-amplified with the same efficiency as the endogenous transcript of interest. By Renée M. Horner.
Details
Details of this protocol, Competitive RT-PCR: Preparation Of Competitor RNA (Subscription Required), are located on a web site other than Biocompare Protocols.