Overview
Laser capture microdissection (LCM) uses an infrared laser, incorporated within a standard microscope, to isolate specific cells or tissues from samples mounted on microscope slides. The samples are viewed through a thermoplastic film that is attached to a microcentrifuge tube lid. Localized heat, caused by the application of a laser pulse, fuses the membrane to the cells of interest, which can then be harvested for further analysis. RNA and proteins can be purified from the isolated cells, allowing detailed analysis of gene expression. By Motoko Morimoto, Masahiro Morimoto, Jeanette Whitmire, Robert A. Star, Joseph F. Urban, Jr. and William C. Gause.
Details
Details of this protocol, Laser Capture Microdissection (LCM): Preparation And Sectioning Of Frozen Tissue Blocks And Purification Of RNA From Isolated Cells (Subscription Required), are located on a web site other than Biocompare Protocols.