Overview
In this method, an oligodeoxynucleotide primer hybridized to mRNA is extended by an RNA-dependent DNA polymerase to create a cDNA copy that can be amplified by PCR. Depending on the purpose of the experiment, the primer for first-strand cDNA synthesis can be specifically designed to hybridize to a particular target gene, or a general primer such as oligo(dT) can be used to prime cDNA synthesis from essentially all mammalian mRNAs. By Joseph Sambrook and David W. Russell
Details
Details of this protocol, Amplification Of CDNA Generated By Reverse Transcription Of MRNA (Subscription Required), are located on a web site other than Biocompare Protocols.