Overview
DNA prepared by PCR-mediated gene disruption can be used to transform yeast in gene replacement experiments. This protocol uses two primers, tailed with approximately 50 nucleotides homologous to the gene of interest, that target insertion of the PCR product to that locus. By David C. Amberg, Daniel J. Burke and Jeffrey N. Strathern.
Details
Details of this protocol, PCR-Mediated Gene Disruption: One-Step Method (Subscription Required), are located on a web site other than Biocompare Protocols.