Overview
The top priority of mutagenic PCR is to introduce the various types of mutations in an unbiased fashion rather than to achieve a high overall level of amplification. Thirty cycles of PCR are carried out to ensure that mismatched termini have ample opportunity to become extended to produce complete copies. By R. Craig Cadwell and Gerald F. Joyce.
Details
Details of this protocol, Mutagenic PCR (Subscription Required), are located on a web site other than Biocompare Protocols.