Overview
This protocol describes how (1) to clone cloned sequences encoding open reading frames in plasmids carrying bacteriophage {lambda} pL promoters, (2) to optimize expression of target proteins in transformants carrying these recombinants, and (3) to scale-up production of foreign proteins.
Joseph Sambrook and David W. Russell
Details
Details of this protocol, Expression Of Cloned Genes In E. Coli Using The Bacteriophage {lambda} PL Promoter (Subscription Required), are located on a web site other than Biocompare Protocols.