Rapid And Efficient Site-Directed Mutagenesis By The Single-Tube Megaprimer PCR Method (Subscription Required) Protocol

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Title	Rapid And Efficient Site-Directed Mutagenesis By The Single-Tube Megaprimer PCR Method (Subscription Required)
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot3467

Overview

The "megaprimer" method of site-directed mutagenesis introduced by Kammann et al. (1989) uses three oligonucleotide primers and two rounds of PCR. By Joseph Sambrook and David W. Russell.

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