Overview
This stage presents a basic plan for capturing a series of images by fluorescent lifetime imaging microscopy. The protocol specifically describes data acquisition for a particular variant of GFP (EGFP) or Oregon Green as a donor fluorophore, but it can be adapted for image acquisition of other chromophore systems as described in the table below. This protocol was provided by Alisa G. Harpur and Philippe I.H. Bastiaens (Imperial Cancer Research Fund, London).
Details
Details of this protocol, Probing Protein Interactions Using GFP And FRET- Stage 3: FLIM-FRET Measurements (Subscription Required), are located on a web site other than Biocompare Protocols.