Overview
This protocol uses FAM-(6-carboxy-fluorescein) or JOE-(6-carboxy-4', 5' -dichloro-2',7' -dimethoxy-fluorescein) labeled LUX (Light Upon eXtension) primers, which can quantify 100 or fewer copies of the target DNA in a background of nonspecific templates, over a broad dynamic range of less than 100-107 copies. By Wolfgang Kusser, Sandrine Javorschi and Martin A. Gleeson.
Details
Details of this protocol, Real-Time PCR (Subscription Required), are located on a web site other than Biocompare Protocols.