Overview
In this protocol, sample and competitor RNAs are reverse transcribed (separately) in a pilot experiment. A constant amount of sample RT product is then combined with a 2-logserial dilution of competitor RT product for PCR. This procedure provides an approximate copy number for the sample, which is then fine-tuned by repeating the experiment with a series of twofold dilutions of competitor. By Renée M. Horner.
Details
Details of this protocol, Competitive RT-PCR: Estimation Of Copy Number (Subscription Required), are located on a web site other than Biocompare Protocols.