Overview
This protocol describes a method for microinjecting proteins into the nucleus or cytoplasm of adherent cells. Microinjection equipment can be obtained from a number of suppliers; this protocol has been used with the Narishige IM-200 air pressure regulator and the Leitz micromanipulator. Using this system, it is possible to microinject a constant volume within a 50% difference among cells.
Yulia Komarova, John Peloquin, and Gary Borisy
Details
Details of this protocol, Microinjection Of Protein Samples (Subscription Required), are located on a web site other than Biocompare Protocols.