Overview
Intermediate filaments (IF) are major cytoskeletal systems of vertebrate and many nonvertebrate cells whose expression is cell-type specific and developmentally regulated. This protocol describes a method for purifying one type of IF, vimentin, from bovine lens tissue. Purification of human vimentin expressed in Escherichia coli is also described. These methods are useful in the preparation of other IF protein subunits for microinjection studies as well.
Edward R. Kuczmarski and Robert D. Goldman
Details
Details of this protocol, Purification Of Bovine Lens And Bacterially Expressed Human Vimentin (Subscription Required), are located on a web site other than Biocompare Protocols.