Detection Of Mycoplasma In Mammalian Cell Cultures Using Fluorescence Microscopy (Subscription Required) Protocol

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Title	Detection Of Mycoplasma In Mammalian Cell Cultures Using Fluorescence Microscopy (Subscription Required)
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot4351

Overview

This procedure describes a method for mycoplasma detection using Hoechst 33258, a fluorescent dye that stains DNA. Staining of particulate DNA matter around the cell nucleus (on the cell surface or in the cytoplasm) is indicative of mycoplasma contamination.

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