Overview
This protocol provides methods for cryofreezing and subsequent thawing of mammalian cells. Pre-confluent cells are trypsinized, pelleted, resuspended in freezing medium, and gradually frozen. When needed, frozen cells are thawed quickly under running tap water and transferred to growth medium.
Details
Details of this protocol, Cryopreservation Of Mammalian Culture Cells: Preparation And Recovery Of Samples (Subscription Required), are located on a web site other than Biocompare Protocols.