Overview
This protocol provides a method for synchronizing cells at the G1/S border using a double treatment of thymidine, which, in excess, is an inhibitor of DNA synthesis. Cells are treated once with excess thymidine to accumulate the majority of them at G1/S; however, some cells will have stopped growth within the S phase.
Details
Details of this protocol, G1/S Phase Synchronization Using Double Thymidine Synchronization (Subscription Required), are located on a web site other than Biocompare Protocols.