Overview
In this protocol, DNA for analysis is purified using salt precipitation. The method is gentle, limits the breakage of the long chromosomal strands, and avoids the use of phenol and chloroform. It is suitable for use with cultured cells, breast tumor tissue that has been subjected to hormone receptor analysis, and blood samples. The loss of heterozygosity assay is performed using a multiplex PCR, in which one of each primer pair is labeled with a different fluorophor. By Lise Hansen and Just Justesen.
Details
Details of this protocol, Loss Of Heterozygosity: A Multiplex PCR Method To Define A Narrow Deleted Chromosomal Region Of A Tumor Genome (Subscription Required), are located on a web site other than Biocompare Protocols.