Overview
This protocol provides a qualitative method for assessing cell death by detecting DNA fragments using agarose gel electrophoresis. One of the classic features of apoptosis is the cleavage of the genomic DNA into oligonucleosomal fragments represented by multiples of 180-200 bp. Visualizing these fragments can aid in characterizing an apoptotic event. This method should always be combined with more quantitative methods in order to compare the degree of apoptosis among the experimental samples.
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel and Douglas R. Green
Details
Details of this protocol, Analysis Of DNA Fragmentation Using Agarose Gel Electrophoresis (Subscription Required), are located on a web site other than Biocompare Protocols.