Overview
This protocol is for bidirectional, blunt-end cloning of DNA fragments. The target DNA is PCR amplified and 3'-extensions are polished with Pfu DNA polymerase. The amplicon is ligated to a blunt-ended plasmid DNA, and the products of the ligation reaction are used to transform competent Escherichia coli. A restriction enzyme is added to the ligation reaction (Liu and Schwartz 1992) to relinearize any self-religating vector DNA. By Gina L. Costa and Michael P. Weiner.
Details
Details of this protocol, Bidirectional Cloning Of PCR Products (Subscription Required), are located on a web site other than Biocompare Protocols.